Flip-flop of glycosylphosphatidylinositols (GPI's) across the ER.
نویسندگان
چکیده
The transbilayer flip-flop of early intermediates in the glycosylphosphatidylinositol (GPI) biosynthetic pathway has been demonstrated using novel fluorescent GPI probes and a biochemical reconstitution approach.
منابع مشابه
Flip - flop of glycosylphosphatidylinositols ( GPI ’ s ) across the ER Ram
General Experimental Procedures: Egg phosphatidylcholine (ePC) and routine chemicals were obtained from Sigma Chemical Co. SM-2 Bio-beads were purchased from Bio-Rad Laboratories. ULTROL grade Triton X-100 was from Roche Molecular Biochemicals. 1-acyl-2-C6-NBD-PC (NBD-PC) was from Avanti Polar Lipids. Salt-washed endoplasmic reticulum (SWER) was prepared from a rat liver homogenate as previousl...
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ورودعنوان ژورنال:
- Chemical communications
دوره 4 شماره
صفحات -
تاریخ انتشار 2005